UNSW Embryology

Human Menstrual Cycle Smears

Secretory


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Day 15-22

Secretory

stratum spinosum cells (blue) which are folded or with curled edges, appear immediately after ovulation due to increase in progesterone. Leukocytes (small black cells) becoming more numerous.

Human Vaginal Smears

Stratum corneum (eosinophilic, red) and stratum spinosum (basophilic, blue) cells throughout the menstrual cycle.

Observe cell size, proportion of pyknotic nuclei, stain intensity and cell morphology. Leukocyte number is variable, but not a reliable criteria.