LIVER AND GALLBLADDER LABORATORY

GOALS AND OBJECTIVES:

1. Identify the hepatocyte cell plates, sinusoids, portal tracts, central veins.
2. Identify Kupffer cells and the location of the space of Disse.
3. Identify the mucosa, muscularis and serosa of the gallbladder.

Slide #42-LIVER

1. Identify the cell plates of hepatocytes. What are the sinusoids? What type of cells line the sinusoids? How many cells thick is the cell plates in adults?
2. Identify the portal tract. The portal triad consists of the bile duct, the branch of the hepatic artery, and a branch of the portal vein. The portal tract includes these plus the connective tissue, lymphatics, nerves, and inflammatory cells.
3. Identify Glisson's capsule.
4. Identify the sinusoids. What cells line the sinusoids? Where is the space of Disse?

Slide #145-LIVER

1. This is a trichrome stain of the liver the connective tissue is stained blue. Identify the portal tracts and structures within the portal tracts. Note the large amount of connective tissue in the portal tracts.

Slide #143-GALLBLADDER

1. What is the mucosal lining cell of the gallbladder. Identify the mucosa, muscularis and serosa or adventia.
2. What is the function of the gallbladder?
3. Review the route of bile from the liver to the gallbladder.