MICROSCOPIC SLIDES - HISTOLOGYIMMUNE SYSTEM I & II

LYMPH NODE

Slide # 87 - Human lymph node - Good delineation of reticular and capsular structure.

Slide #87

• Capsule
• Subcapsular sinusoids
• Cortex - medulla
• Germinal centers and primary follicles
• Medullary cords
• Medullary sinusoids - lymphatic fluid
• Pigmented macrophages (carbon)
• Subcortical sinusoids
• Paracortex poorly developed

Slide #96

• Paracortex clearly seen
• Describe germinal center cells
• High endothelial venules (compare to vessels with flattened endothelium).
• Large granular macrophages and eosinophils in sinusoids = sinus histiocytosis.

Spleen

Slide #193 - Monkey spleen

Slide #191 - Reticular stain

Slide #193

• Red pulp and white pulp
• How do these zones compare to cortex and medulla of lymph node?
• Red pulp

1. Cords
2. Sinusoids - Why are contents of spleen sinusoids different from lymph node sinusoids?

• White pulp

1. Periarteriolar zone
2. Follicle
3. Mantle
4. Marginal zone

1. Structure of germinal center cells
2. Name four types of macrophage lineage cells in the spleen.

Slide #94

• Why is white pulp less defined?
• What is significance of acellular zones in white pulp?

Slide #191

• Reticular stain outlines sinusoids.
• Splenic cords contain reticular fibers.

GALT

Slide #34 - Appendix

Slide #33 - Ileum

Slide #26 - Esophagus

Slide #93 - Tonsil

• Stratified squamous epithelium
• Interepithelial lymphocytes
• Prominent follicles and mantles ***Note mitoses in follicles
• Interfollicular T cell zones

• Dense capsule
• Skeletal muscle
• Salivary glands
• Draining lymphatics
• Nerves

Slide #34 -Appendix - relatively acellular, variation of normal.

• Columnar epithelium
• Follicles, mantle, T cell zones
• Lacteals
• Draining lymphatics

Slide #33 -Ileum, (Peyer's Patch)

• Submucosa - diffuse
• Lymphocytes, macrophages, plasma cells
• Lacteals and capillaries in villae
• Follicles and T cell zones
• Specialized epithelium overlying Peyer's Patch not easily seen!

Slide #26 -Esophagus and Stomach

• Diffuse subepithelial lymphoid tissue
• Some intraepithelial lymphocytes
• Note heterogenity of cells; prominant plasma cells in gastric mucosa.

THYMUS

Slide #95 - (Young)

• Note lobular structure
• Cortex and medulla
• Cortex Small lymphocytes, Thymic nurse cells.

1. Spaces - "Starry sky" - cellular "debris" - What does this represent?

• Medulla -

1. Hassalls corpuscles
2. Epithelial stroma,

• No follicles, no B cells, no plasma cells!