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E3-E6: Small intestine. Loss of ventral attachment of mesentery to body wall as the two canals of I-E coelom open to the extra-embryonic coelom (chorionic sac). In E5 and E6, vitelline duct of yolk sac stalk is cut close to its attachment to the yolk sac in the embryo-embryonic coelom (yolk sac removed).
E4-E6: Junction of hindgut and urogenital sinus (whole region called cloaca).
DEVELOPMENT OF THE NERVOUS SYSTEM
E4: Spinal cord has enlarged ventral horns (motor neurons).
DEVELOPMENT OF THE MUSCULOSKELETAL SYSTEM
E5: Hindlimb and Forelimb folds.